Overview

  • Product name

    Anti-NCS1 antibody [EPR7699]
    See all NCS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7699] to NCS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NCS1 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • U-87 MG, SH-SY5Y, A549, 293T and Human fetal brain lysates; Human brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7699
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
  • Sequence similarities

    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasm > perinuclear region. Cell membrane. Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9430075O15Rik antibody
    • A730032G13Rik antibody
    • AI836659 antibody
    • DKFZp761L1223 antibody
    • FLUP antibody
    • FREQ antibody
    • Frequenin antibody
    • Frequenin homolog (Drosophila) antibody
    • Frequenin homolog antibody
    • Frequenin like protein antibody
    • Frequenin, Drosophila, homolog of antibody
    • Frequenin-like protein antibody
    • Frequenin-like ubiquitous protein antibody
    • Mfreq antibody
    • NCS 1 antibody
    • NCS-1 antibody
    • ncs1 antibody
    • NCS1_HUMAN antibody
    • Neuronal calcium sensor 1 antibody
    • Neuronal Calicum Sensor 1 antibody
    see all

Images

  • All lanes : Anti-NCS1 antibody [EPR7699] (ab129166) at 1/1000 dilution

    Lane 1 : U-87 MG cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : A549 cell lysates
    Lane 4 : 293T cell lysates
    Lane 5 : Fetal brain tissue lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • Overlay histogram showing SH-SY5Y cells stained with ab129166 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129166, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab129165, at 1/250 dilution staining NCS1 in paraffin-embedded Human brain tissue, by Immunohistochemistry.

References

This product has been referenced in:

  • Uchida A  et al. Involvement of dual-strand of the miR-144 duplex and their targets in the pathogenesis of lung squamous cell carcinoma. Cancer Sci 110:420-432 (2019). Read more (PubMed: 30375717) »
  • Moore LM  et al. An assessment of neuronal calcium sensor-1 and response to neoadjuvant chemotherapy in breast cancer patients. NPJ Breast Cancer 4:6 (2018). ICC/IF . Read more (PubMed: 29560416) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (B16F10 melanoma and Mouse Fibroblast)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
B16F10 melanoma and Mouse Fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Brian Koss

Verified customer

Submitted Oct 11 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (WM266 melanoma and HEK293T)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
WM266 melanoma and HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 11 2018

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