Product nameAnti-NCX1 antibody
See all NCX1 primary antibodies
DescriptionRabbit polyclonal to NCX1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse
Synthetic peptide corresponding to Mouse NCX1 aa 650-750 conjugated to keyhole limpet haemocyanin.
Database link: P70414
- This antibody gave a positive signal in both Mouse and Rat Heart tissue lysate within WB. IHC-P (FFPE): Mouse Kidney (Normal)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 108 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
Tissue specificityExpressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung.
Sequence similaritiesBelongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.
Contains 2 Calx-beta domains.
Cellular localizationCell membrane.
- Information by UniProt
- CNC antibody
- DKFZp779F0871 antibody
- MGC119581 antibody
All lanes : Anti-NCX1 antibody (ab151608) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate (ab27255)
Lane 2 : Heart (Rat) Tissue Lysate (ab54976)
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible non-specific binding), 68 kDa (possible non-specific binding)
Exposure time: 30 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151608 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
IHC image of ab151608 staining in Mouse Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab151608, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Bai T et al. Resveratrol protects against lipopolysaccharide-induced cardiac dysfunction by enhancing SERCA2a activity through promoting the phospholamban oligomerization. Am J Physiol Heart Circ Physiol 311:H1051-H1062 (2016). Read more (PubMed: 27591219) »
- Long Z et al. The reverse-mode NCX1 activity inhibitor KB-R7943 promotes prostate cancer cell death by activating the JNK pathway and blocking autophagic flux. Oncotarget 7:42059-42070 (2016). Read more (PubMed: 27275542) »