Key features and details
- Mouse monoclonal [9E4-2D8] to ND4 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-ND4 antibody [9E4-2D8] - N-terminal
DescriptionMouse monoclonal [9E4-2D8] to ND4 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ND4 (N terminal).
Database link: P03905
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
This product was previously labelled as NADH dehydrogenase subunit 4
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride
Concentration information loading...
PurityProtein L purified
Purification notesPurified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG).
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab219822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).
Western blot using whole cell extracts is not recommended.
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Involvement in diseaseDefects in MT-ND4 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ND4 are a cause of Leber hereditary optic neuropathy with dystonia (LDYT) [MIM:500001]; also called familial dystonia with visual failure and striatal lucencies. LDYT is part of a spectrum of Leber hereditary optic neuropathy. It is characterized by the association of optic atrophy and central vision loss with dystonia.
Defects in MT-ND4 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
Sequence similaritiesBelongs to the complex I subunit 4 family.
Cellular localizationMitochondrion membrane.
- Information by UniProt
- MT-ND4 antibody
- MTND4 antibody
- NADH dehydrogenase subunit 4 antibody
All lanes : Anti-ND4 antibody [9E4-2D8] - N-terminal (ab219822) at 4 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219822 has been referenced in 1 publication.
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134