Overview

  • Product name

    Anti-ND4 antibody [9E4-2D8] - N-terminal
  • Description

    Mouse monoclonal [9E4-2D8] to ND4 - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ND4 (N terminal).
    Database link: P03905

  • Positive control

    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).
  • General notes

    Western blot advice:

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

     This product was previously labelled as NADH dehydrogenase subunit 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab219822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).

Western blot using whole cell extracts is not recommended.

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease

    Defects in MT-ND4 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-ND4 are a cause of Leber hereditary optic neuropathy with dystonia (LDYT) [MIM:500001]; also called familial dystonia with visual failure and striatal lucencies. LDYT is part of a spectrum of Leber hereditary optic neuropathy. It is characterized by the association of optic atrophy and central vision loss with dystonia.
    Defects in MT-ND4 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
  • Sequence similarities

    Belongs to the complex I subunit 4 family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MT-ND4 antibody
    • MTND4 antibody
    • NADH dehydrogenase subunit 4 antibody
    • NADH-ubiquinone oxidoreductase chain 4 antibody
    • NADH4 antibody
    • ND4 antibody
    • NU4M_HUMAN antibody
    see all

Images

  • All lanes : Anti-ND4 antibody [9E4-2D8] - N-terminal (ab219822) at 4 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled Goat-anti-mouse IgG

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References

ab219822 has not yet been referenced specifically in any publications.

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