Key features and details
- Rabbit polyclonal to ND6
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ND6 antibody
See all ND6 primary antibodies
DescriptionRabbit polyclonal to ND6
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Human breast cancer and rat brain tissues.
This product was previously labelled as NADH Dehydrogenase subunit 6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab214224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceNADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight.
Cellular localizationMitochondrion membrane; Multi-pass membrane protein.
- Mitochondrially encoded NADH dehydrogenase 6 antibody
- MT ND6 antibody
- mtND6 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling ND6 with ab214224 at 1/100 dilution, followed by secondary antibody detection and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ND6 with ab214224 at 1/400 dilution, followed by secondary antibody detection and DAB staining.
ab214224 has not yet been referenced specifically in any publications.