Western blot abreview for Anti-NADH Dehydrogenase subunit 6 antibody

Below Average
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole larval extract)
Loading amount
100 µg
Specification
Whole larval extract
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST+5% milk

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/500

Detection

Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: None kDa Non-specific: 33, 34, 39, 60 kDa

Additional data

Additional Notes
Marker (M) and larval extract (1-3).
Abcam response
Thank you for your valuable feedback. ab81212 had not been previously tested in Drosophila tissue and your data suggests that ab81212 may not detect NADH Dehydrogenase subunit 6 under these conditions. We hope this information is useful to our customers.

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Submitted Nov 05 2012

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