• Product name

  • Description

    Rabbit polyclonal to NDEL1
  • Host species

  • Specificity

    ab25959 recognises NDEL1.

  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human NDEL1.
    (Peptide available as ab39840)

  • Positive control

    • Jurkat whole cell lysate (ab7899).
  • General notes

     This product was previously labelled as Nudel




Our Abpromise guarantee covers the use of ab25959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. PubMed: 17984054
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.


  • Function

    Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the nudE family.
  • Developmental stage

    Expression peaks in mitosis.
  • Post-translational

    Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • A. nidulans antibody
    • DKFZp451M0318 antibody
    • EOPA antibody
    • MITAP 1 antibody
    • MITAP1 antibody
    • Mitosin associated protein 1 antibody
    • Mitosin associated protein MITAP1 antibody
    • Mitosin-associated protein 1 antibody
    • Ndel 1 antibody
    • NDEL1 antibody
    • NDEL1_HUMAN antibody
    • Nuclear distribution gene E like homolog 1 antibody
    • Nuclear distribution protein nudE like 1 antibody
    • Nuclear distribution protein nudE-like 1 antibody
    • NUDE like protein antibody
    • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
    • NudE nuclear distribution gene E homolog like 1 antibody
    • NUDEL antibody
    • Protein Nudel antibody
    see all


  • Lane 1 : Anti-NDEL1 antibody (ab25959) at 0.5 µg/ml
    Lane 2 : Anti-NDEL1 antibody (ab25959) at 1 µg/ml
    Lane 3 : Anti-NDEL1 antibody (ab25959) at 2 µg/ml

    All lanes : Jurkat whole cell lysate.

    Predicted band size: 38 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

  • ab25959 at 2µg/ml staining NDEL1 in Jurkat cells by ICC/IF

  • Immunofluorescence of NDEL1 in Jurkat cells using ab25959 at 10 ug/ml.


This product has been referenced in:

  • Chapman DE  et al. Regulation of in vivo dynein force production by CDK5 and 14-3-3e and KIAA0528. Nat Commun 10:228 (2019). Read more (PubMed: 30651536) »
  • Turgay Y  et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . Read more (PubMed: 24662567) »
See all 6 Publications for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (U2OS and Hela)
U2OS and Hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%


Verified customer

Submitted Dec 01 2006

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