Key features and details
- Rabbit polyclonal to Ndfip1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ndfip1 antibody
See all Ndfip1 primary antibodies
DescriptionRabbit polyclonal to Ndfip1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Ndfip1 aa 2-116.
Database link: Q9BT67
- WB: HEK-293T and HeLa whole cell lysate (ab150035). IHC-P: Human breast cancer and adrenal gland tissue. ICC/IF: HEK-293 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionActivates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.
Tissue specificityWidely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.
DomainThe PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase binding and activation and for ubiquitination.
modificationsUbiquitinated by NEDD4 and ITCH; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation.
Undergoes transient tyrosine phosphorylation following EGF stimulation, most probably by catalyzed by SRC. Phosphorylation SRC is enhanced in the presence of NDFIP2 which may act as a scaffold to recruit SRC to NDFIP1.
Cellular localizationEndosome membrane. Golgi apparatus membrane. Secreted. Detected in exosomes and secreted via the exosomal pathway.
- Information by UniProt
- 0610010M22Rik antibody
- Breast cancer associated protein SGA 1M antibody
- Breast cancer-associated protein SGA-1M antibody
All lanes : Anti-Ndfip1 antibody (ab236892) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
Paraffin-embedded human breast cancer tissue stained for Ndfip1 using ab236892 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for Ndfip1 using ab236892 at 1/100 dilution in immunohistochemical analysis.
HEK-293 (human epithelial cell line from embryonic kidney) cells labeling Ndfip1 using ab236892 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-copnjugated goat anti-rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236892 has not yet been referenced specifically in any publications.