Key features and details
- Mouse polyclonal to NDFIP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NDFIP2 antibody
DescriptionMouse polyclonal to NDFIP2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
NDFIP2 full-length human protein
- NDFIP2 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionActivates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.
Tissue specificityExpressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.
DomainThe PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination.
modificationsUbiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover.
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN.
Cellular localizationEndosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane.
- Information by UniProt
- Ndfip2 antibody
- NEDD4 family interacting protein 2 antibody
- NEDD4 family-interacting protein 2 antibody
All lanes : Anti-NDFIP2 antibody (ab67912) at 1/500 dilution
Lane 1 : NDFIP2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
ab67912 has not yet been referenced specifically in any publications.