Key features and details
- Rabbit polyclonal to NDP52
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NDP52 antibody
See all NDP52 primary antibodies
DescriptionRabbit polyclonal to NDP52
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human NDP52 aa 174-389.
Database link: Q13137
- IHC-P: H441 xenograft. WB: Raji cells, Hu7 whole cell extract. ICC/IF: HeLa cells. IP: Jurkat cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/10000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
FunctionMay play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.
Tissue specificityExpressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
- Information by UniProt
- Antigen nuclear dot 52 kDa protein antibody
- CACO2_HUMAN antibody
- Calcium binding and coiled coil domain 2 antibody
NDP52 was immunoprecipitated from Jurkat whole cell extracts with 5 μg ab151256. Western blot was performed on the immunoprecipitate using ab151256. EasyBlot anti-rabbit IgG was used as the secondary antibody.
All lanes : Anti-NDP52 antibody (ab151256) at 1/2000 dilution
Lane 1 : Untreated Huh-7 whole cell extracts
Lane 2 : Huh-7 whole cell extracts treated with Thapsigargin for 12hrs
Lane 3 : Huh-7 whole cell extracts treated with Thapsigargin for 24hrs
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
HeLa stained for NDP52 (green) using ab151256 at 1/1000 dilution in ICC/IF.
Immunohistochemical analysis of paraffin embedded H441 xenograft labeling NDP52 with ab151256 antibody at 1/100.
Anti-NDP52 antibody (ab151256) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
ab151256 has not yet been referenced specifically in any publications.