Product nameAnti-NDP52 antibody
See all NDP52 primary antibodies
DescriptionRabbit polyclonal to NDP52
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human NDP52 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human Lysates: TE 671 Whole Cell, HeLa Whole Cell - Hydroxyurea Treated (48hr, 2µM), Ramos Whole Cell, Placenta Tissue ICC-IF: Hela cells untreated, Hela cells chloroquine treated.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab68588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
|IP||Use at an assay dependent concentration. PubMed: 23209807|
FunctionMay play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.
Tissue specificityExpressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
- Information by UniProt
- Antigen nuclear dot 52 kDa protein antibody
- CACO2_HUMAN antibody
- Calcium binding and coiled coil domain 2 antibody
ab68588 staining NDP52 in HeLa cells +/- Chloroquine (50μM, 24 hours). The cells were fixed with 4% paraformaldheyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab68588 at 5μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. The secondary antibodies were ab150081 (colored green) and ab150120 (pseudo-colored red) used at 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.
All lanes : Anti-NDP52 antibody (ab68588) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
IHC image of NDP52 staining in Human Cerebral Cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68588, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
This product has been referenced in:
- Ravenhill BJ et al. The Cargo Receptor NDP52 Initiates Selective Autophagy by Recruiting the ULK Complex to Cytosol-Invading Bacteria. Mol Cell 74:320-329.e6 (2019). Read more (PubMed: 30853402) »
- Baillet N et al. Autophagy Promotes Infectious Particle Production of Mopeia and Lassa Viruses. Viruses 11:N/A (2019). Read more (PubMed: 30909570) »