Key features and details
- Rabbit polyclonal to NDRG1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NDRG1 antibody
See all NDRG1 primary antibodies
DescriptionRabbit polyclonal to NDRG1
SpecificityThe antibody detects endogenous level of total NDRG1 protein.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human NDRG1 (C terminal).
Database link: Q92597
- U251, HeLa, SW480, PC3, mouse kidney,eye and kidney cell extracts. Mouse lung, rat brain and rat kidney tissue. MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionMay have a growth inhibitory role.
Tissue specificityUbiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Involvement in diseaseDefects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
Sequence similaritiesBelongs to the NDRG family.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
- Information by UniProt
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
All lanes : Anti-NDRG1 antibody (ab196621) at 1/50 dilution
Lane 1 : U251 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SW480 cell extract
Lane 4 : PC3 cell extract
Lane 5 : Mouse kidney cell extract
Lane 6 : Mouse eye cell extract
Lane 7 : Mouse brain cell extract
Developed using the ECL technique.
Predicted band size: 43 kDa
Immunofluorescence analysis of MCF7 cells labeling NDRG1 with ab196621 at a 1/50 dilution. Counter-stained with DAPI (blue) for nuclear staining.
Immunohistochemical analysis of formalin fixed and paraffin embedded rat kidney tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
Immunohistochemical analysis of formalin fixed and paraffin embedded rat lung tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
ab196621 has been referenced in 1 publication.
- Lee E et al. Reduction of two histone marks, H3k9me3 and H3k27me3 by epidrug induces neuroendocrine differentiation in prostate cancer. J Cell Biochem 119:3697-3705 (2018). PubMed: 29236331