Overview

  • Product name

  • Description

    Rabbit polyclonal to NDRG1
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous level of total NDRG1 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human NDRG1 (C terminal).
    Database link: Q92597

  • Positive control

    • U251, HeLa, SW480, PC3, mouse kidney,eye and kidney cell extracts. Mouse lung, rat brain and rat kidney tissue. MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    May have a growth inhibitory role.
  • Tissue specificity

    Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • Involvement in disease

    Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • Sequence similarities

    Belongs to the NDRG family.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt
  • Database links

  • Alternative names

    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

Images

  • All lanes : Anti-NDRG1 antibody (ab196621) at 1/50 dilution

    Lane 1 : U251 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SW480 cell extract
    Lane 4 : PC3 cell extract
    Lane 5 : Mouse kidney cell extract
    Lane 6 : Mouse eye cell extract
    Lane 7 : Mouse brain cell extract

    Developed using the ECL technique.

    Predicted band size: 43 kDa

  • Immunofluorescence analysis of MCF7 cells labeling NDRG1 with ab196621 at a 1/50 dilution. Counter-stained with  DAPI (blue) for nuclear staining.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded rat kidney tissue labeling NDRG1 with ab196621 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling NDRG1 with ab196621 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded rat lung tissue labeling NDRG1 with ab196621 at a 1/100 dilution.

References

This product has been referenced in:

  • Lee E  et al. Reduction of two histone marks, H3k9me3 and H3k27me3 by epidrug induces neuroendocrine differentiation in prostate cancer. J Cell Biochem 119:3697-3705 (2018). Read more (PubMed: 29236331) »
See 1 Publication for this product

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