Anti-NDRG1 antibody (ab37897)
Key features and details
- Goat polyclonal to NDRG1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDRG1 antibody
See all NDRG1 primary antibodies -
Description
Goat polyclonal to NDRG1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide:
GNSAGPKSMEVSC
, corresponding to C terminal amino acids 382-394 of Human NDRG1 -
Positive control
- Recombinant human NDRG1 protein (ab87685) can be used as a positive control in WB. Human Kidney, Cerebellum and Testis tissue lysates; IHC-P: Human Prostate, hepatocellular carcinoma and colon tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa.
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 0.5 - 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa. 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 0.5 - 2.5 µg/ml. |
Target
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Function
May have a growth inhibitory role. -
Tissue specificity
Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression. -
Involvement in disease
Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. -
Sequence similarities
Belongs to the NDRG family. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro. - Information by UniProt
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Database links
- Entrez Gene: 10397 Human
- Entrez Gene: 17988 Mouse
- Entrez Gene: 299923 Rat
- Omim: 605262 Human
- SwissProt: Q92597 Human
- SwissProt: Q62433 Mouse
- SwissProt: Q6JE36 Rat
- Unigene: 372914 Human
see all -
Alternative names
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (33)
ab37897 has been referenced in 33 publications.
- Panigrahi SK et al. FOXP1 and NDRG1 act differentially as downstream effectors of RAD9-mediated prostate cancer cell functions. Cell Signal 86:110091 (2021). PubMed: 34298089
- Ho JJD et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism. Nat Commun 11:2677 (2020). PubMed: 32472050
- Balukoff NC et al. A translational program that suppresses metabolism to shield the genome. Nat Commun 11:5755 (2020). PubMed: 33188200
- Park KC et al. Thiosemicarbazones suppress expression of the c-Met oncogene by mechanisms involving lysosomal degradation and intracellular shedding. J Biol Chem 295:481-503 (2020). PubMed: 31744884
- Ogasawara R et al. Rapamycin and mTORC2 inhibition synergistically reduce contraction-stimulated muscle protein synthesis. J Physiol 598:5453-5466 (2020). PubMed: 32893874