Overview

  • Product name

  • Description

    Rabbit polyclonal to NDRG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C-terminal region of mouse NDRG1

Properties

Applications

Our Abpromise guarantee covers the use of ab63989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/400.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    May have a growth inhibitory role.
  • Tissue specificity

    Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • Involvement in disease

    Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • Sequence similarities

    Belongs to the NDRG family.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt
  • Database links

  • Alternative names

    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

Images

  • ab63989 at 1/400 dilution staining NDRG1 in human placenta and placental membrane by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to incubating with ab63989 for 16 minutes at 25°C. A HRP conjugated goat polyclonal to mouse Ig was used as the secondary antibody.

    See Abreview

  • Anti-NDRG1 antibody (ab63989) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    NDRG1 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Du A  et al. NDRG1 Downregulates ATF3 and Inhibits Cisplatin-Induced Cytotoxicity in Lung Cancer A549 Cells. Int J Med Sci 15:1502-1507 (2018). Read more (PubMed: 30443171) »
  • Costin BN  et al. Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse prefrontal cortex. PLoS One 8:e72979 (2013). WB . Read more (PubMed: 23991167) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us. While the entire sequence of NDRG1 may be similar to NDRG4, the immmunogenic peptide used for this antibody has only 58% homology with the NDRG sequence and is not likely to react. Generally above 85% there is likely reactivity. There is very low homology with the other NRDG sequences as well and the antibody should be specific for NDRG1.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for contacting Abcam regarding ab63989.


Below please find theprotocol details you requested:


Gel type: 4-12% Bis-tris gel using MOPS buffer system


Sample preparation: Lysates prepared from liquid nitrogen fresh frozen tissues in RIPA buffer




I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for contacting Abcam.

As the NDRG1 has a molecular weight of around 50kDa, I would recommend running your samples on a 12.5% SDS gel.

We do have a number of precast gels in our catalogue that you may be interested in, and there is a link to those below:

https://www.abcam.com/index.html?pageconfig=resource&rid=14719



If there is anything else I can help you with, please let me know.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta and placental membrane)
Specification
placenta and placental membrane
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, 100C, 20 minutes
Permeabilization
No

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Submitted Sep 11 2009

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