Recombinant
RabMAb

Recombinant Anti-NDRG1 antibody [EPR5593] (Alexa Fluor® 647) (ab199471)

Overview

  • Product name

    Anti-NDRG1 antibody [EPR5593] (Alexa Fluor® 647)
    See all NDRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5593] to NDRG1 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NDRG1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    May have a growth inhibitory role.
  • Tissue specificity

    Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • Involvement in disease

    Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • Sequence similarities

    Belongs to the NDRG family.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt
  • Database links

  • Alternative names

    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

Images

  • ab199471 staining NDRG1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab199471 has not yet been referenced specifically in any publications.

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