Recombinant
RabMAb

Recombinant Anti-NDRG1 antibody [EPR5593] - Low endotoxin, Azide free (ab216457)

Overview

  • Product name

    Anti-NDRG1 antibody [EPR5593] - Low endotoxin, Azide free
    See all NDRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5593] to NDRG1 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids of Human NDRG1.

  • Positive control

    • Caco 2, Human fetal kidney, LnCaP, and HeLa cell lysates; Human colon tissue; HeLa cells.
  • General notes

    ab216457 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May have a growth inhibitory role.
    • Tissue specificity

      Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
    • Involvement in disease

      Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
    • Sequence similarities

      Belongs to the NDRG family.
    • Cellular localization

      Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
    • Information by UniProt
    • Database links

    • Alternative names

      • 42 kDa antibody
      • Anti GC4 antibody
      • cap43 antibody
      • cmt4d antibody
      • Differentiation related gene1 protein antibody
      • Differentiation-related gene 1 protein antibody
      • Drg 1 antibody
      • DRG-1 antibody
      • drg1 antibody
      • gc4 antibody
      • GC4 antibody antibody
      • hmsnl antibody
      • Human mRNA for RTP complete cds antibody
      • N myc downstream regulated gene 1 antibody
      • N myc downstream regulated gene 1 protein antibody
      • N-myc downstream-regulated gene 1 protein antibody
      • Ndr 1 antibody
      • ndr1 antibody
      • NDRG 1 antibody
      • Ndrg1 antibody
      • NDRG1 protein antibody
      • NDRG1_HUMAN antibody
      • Nickel specific induction protein antibody
      • Nickel specific induction protein Cap43 antibody
      • Nickel-specific induction protein Cap43 antibody
      • nmsl antibody
      • Nmyc downstream regulated antibody
      • Nmyc downstream regulated gene1 antibody
      • Nmyc downstream regulated gene1 protein antibody
      • Protein NDRG1 antibody
      • Protein regulated by oxygen 1 antibody
      • Protein regulated by oxygen1 antibody
      • Proxy1 antibody
      • Reduced in tumor antibody
      • Reducin antibody
      • Reducing agents and tunicamycin responsive protein antibody
      • Reducing agents and tunicamycin-responsive protein antibody
      • Rit42 antibody
      • RTP antibody
      • targ1 antibody
      • TDD5 antibody
      • tdds antibody
      • Tunicamycin responsive protein antibody
      see all

    Images

    • ab124689 showing positive staining in Cervical carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • Lane 1 (input): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab124689 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124689 in HeLa whole cell lysate

      Ab124689 (Purified) at 1:500 dilution (1.86 µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.

      Blocking and diluting buffer: 5% NFDM /TBST .

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • ab124689, at 1/100 dilution, staining NDRG1 in HeLa cells by Immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • ab124689 showing positive staining in Colonic adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • ab124689 showing positive staining in Normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • ab124689 showing positive staining in Breast carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

    • This IHC data was generated using the same anti-NDRG1 antibody clone, EPR5593, in a different buffer formulation (cat# ab124689).

      ab124689 at 1/100 dilution, staining NDRG1 in Formalin fixed Paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This ICC/IF data was generated using the same anti-NDRG1 antibody clone, EPR5593, in a different buffer formulation (cat# ab124689).

      ab124689, at 1/100 dilution, staining NDRG1 in HeLa cells by Immunofluorescence.

    References

    ab216457 has not yet been referenced specifically in any publications.

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