Product nameAnti-NDRG1 (phospho S330) antibody [EPR5594]
See all NDRG1 primary antibodies
DescriptionRabbit monoclonal [EPR5594] to NDRG1 (phospho S330)
SpecificityThis antibody only detects NDRG1 when phosphorylated at Serine 330.
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding Serine 330 of Human NDRG1
- Calyculin A treated 293T cell lysates; Human kidney tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 5.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 43 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionMay have a growth inhibitory role.
Tissue specificityUbiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Involvement in diseaseDefects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
Sequence similaritiesBelongs to the NDRG family.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
- Information by UniProt
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
All lanes : Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713) at 1/10000 dilution
Lane 1 : Untreated 293T cell lysates
Lane 2 : Calyculin A treated 293T cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
ab124713, at 1/100, staining NDRG1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Skedsmo FS et al. Cell and context-dependent sorting of neuropathy-associated protein NDRG1 - insights from canine tissues and primary Schwann cell cultures. BMC Vet Res 15:121 (2019). Read more (PubMed: 31029158) »
- Sevinsky CJ et al. NDRG1 regulates neutral lipid metabolism in breast cancer cells. Breast Cancer Res 20:55 (2018). Read more (PubMed: 29898756) »