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Synthetic peptide within Human NDRG2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UN36
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab174850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 41 kDa.|
Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HepG2 cells labeing NDRG2 using ab174850 at 1/10 dilution (red) or a rabbit IgG negative (green).
Immunofluorescent analysis of HepG2 cells labeling NDRG2 using ab174850 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.
ab174850 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"