Overview

  • Product name
    Anti-NDRG2 antibody [EPR11910]
    See all NDRG2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11910] to NDRG2
  • Host species
    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NDRG2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UN36

  • Positive control
    • WB: HepG2, SW620 and SW480 cell lysates, heart, Human brain, Mouse brain, Rat brain tissue lysates; IHC-P: Human fetal kidney, Human astrocytoma; Human skeletal muscle and Human lung carcinoma tissues; FC: HepG2 cells ICC/IF: HepG2 cells. IP: HepG2
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.

Target

  • Function
    May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
  • Tissue specificity
    Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
  • Sequence similarities
    Belongs to the NDRG family.
  • Developmental stage
    Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antidepressant related protein ADRG123 antibody
    • Cytoplasmic protein Ndr1 antibody
    • DKFZp781G1938 antibody
    • FLJ25522 antibody
    • KIAA1248 antibody
    • N myc downstream regulated gene 2 antibody
    • N myc downstream regulator 2 antibody
    • NDR1 related protein NDR2 antibody
    • NDRG 2 antibody
    • NDRG family member 2 antibody
    • NDRG1 related protein antibody
    • NDRG2 antibody
    • NDRG2_HUMAN antibody
    • Protein NDRG2 antibody
    • Protein Syld709613 antibody
    • SYLD antibody
    • Syld709613 protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling NDRG2 with purified ab174850 at 1:100 dilution (2.4 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling NDRG2 with purified ab174850 at 1:100 dilution (2.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • All lanes : Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates
    Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 5 : SW620 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    SW480 and SW620 cells are NDRG2 negative cell lines reported by PMID 23900729

  • ab174850 (purified) at 1:20 dilution (2µg) immunoprecipitating NDRG2 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab174850 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab174850 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling NDRG2 with purified ab174850 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • All lanes : Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow cytometric analysis of permeabilized HepG2 cells labeing NDRG2 using ab174850 at 1/10 dilution (red) or a rabbit IgG negative (green).

  • Immunofluorescent analysis of HepG2 cells labeling NDRG2 using ab174850 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.

References

ab174850 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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