Overview

  • Product name
    Anti-NDRG2 antibody [EPR11910]
    See all NDRG2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11910] to NDRG2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NDRG2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UN36

  • Positive control
    • HepG2 cell lysate; Human fetal kidney; heart and brain tissue lysates; Human astrocytoma; Human skeletal muscle; HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.

Target

  • Function
    May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
  • Tissue specificity
    Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
  • Sequence similarities
    Belongs to the NDRG family.
  • Developmental stage
    Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antidepressant related protein ADRG123 antibody
    • Cytoplasmic protein Ndr1 antibody
    • DKFZp781G1938 antibody
    • FLJ25522 antibody
    • KIAA1248 antibody
    • N myc downstream regulated gene 2 antibody
    • N myc downstream regulator 2 antibody
    • NDR1 related protein NDR2 antibody
    • NDRG 2 antibody
    • NDRG family member 2 antibody
    • NDRG1 related protein antibody
    • NDRG2 antibody
    • NDRG2_HUMAN antibody
    • Protein NDRG2 antibody
    • Protein Syld709613 antibody
    • SYLD antibody
    • Syld709613 protein antibody
    see all

Images

  • Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow cytometric analysis of permeabilized HepG2 cells labeing NDRG2 using ab174850 at 1/10 dilution (red) or a rabbit IgG negative (green).

  • Immunofluorescent analysis of HepG2 cells labeling NDRG2 using ab174850 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.

  • All lanes : Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

References

ab174850 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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