Recombinant Anti-NDRG2 antibody [EPR11910] - BSA and Azide free (ab240181)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11910] to NDRG2 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NDRG2 antibody [EPR11910] - BSA and Azide free
See all NDRG2 primary antibodies -
Description
Rabbit monoclonal [EPR11910] to NDRG2 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This antibody does not cross-react with human NDRG4.
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Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human fetal kidney, Human astrocytoma; Human skeletal muscle and Human lung carcinoma tissues; IP: HepG2
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General notes
ab240181 is the carrier-free version of ab174850.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11910 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-NDRG2 antibody [EPR11910] (ab174850)
- PE Anti-NDRG2 antibody [EPR11910] (ab303175)
- APC Anti-NDRG2 antibody [EPR11910] (ab303176)
- HRP Anti-NDRG2 antibody [EPR11910] (ab303177)
- Alexa Fluor® 594 Anti-NDRG2 antibody [EPR11910] (ab310393)
- Alexa Fluor® 555 Anti-NDRG2 antibody [EPR11910] (ab311919)
- Alexa Fluor® 568 Anti-NDRG2 antibody [EPR11910] (ab312390)
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
Target
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Function
May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity. -
Tissue specificity
Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level). -
Sequence similarities
Belongs to the NDRG family. -
Developmental stage
Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons. - Information by UniProt
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Database links
- Entrez Gene: 57447 Human
- Entrez Gene: 29811 Mouse
- Entrez Gene: 171114 Rat
- Omim: 605272 Human
- SwissProt: Q9UN36 Human
- SwissProt: Q9QYG0 Mouse
- SwissProt: Q8VBU2 Rat
- Unigene: 525205 Human
see all -
Alternative names
- Antidepressant related protein ADRG123 antibody
- Cytoplasmic protein Ndr1 antibody
- DKFZp781G1938 antibody
see all
Images
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All lanes : Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution
Lane 1 : His-tagged human NDRG2 recombinant protein, 10 ng
Lane 2 : His-tagged human NDRG4 recombinant protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174850).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab213204 was used as total protein control at 1/5000 dilution.
This antibody does not cross-react with human NDRG4.
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ab174850 (purified) at 1:20 dilution (2µg) immunoprecipitating NDRG2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab174850 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab174850 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling NDRG2 with purified ab174850 at 1:100 dilution (2.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174850).
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174850).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174850).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240181 has not yet been referenced specifically in any publications.