Recombinant Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-NDRG2 antibody [EPR11911(B)]
See all NDRG2 primary antibodies -
Description
Rabbit monoclonal [EPR11911(B)] to NDRG2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Cynomolgus monkey, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human NDRG2 (UniProt: Q9UN36).
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Positive control
- Human fetal heart, fetal brain, HepG2 or fetal kidney lysate, Human heart or liver tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11911(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Target
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Function
May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity. -
Tissue specificity
Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level). -
Sequence similarities
Belongs to the NDRG family. -
Developmental stage
Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons. - Information by UniProt
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Database links
- Entrez Gene: 100499579 Chimpanzee
- Entrez Gene: 515063 Cow
- Entrez Gene: 57447 Human
- Entrez Gene: 29811 Mouse
- Entrez Gene: 100174656 Orangutan
- Entrez Gene: 171114 Rat
- Entrez Gene: 493281 Xenopus tropicalis
- Omim: 605272 Human
see all -
Alternative names
- Antidepressant related protein ADRG123 antibody
- Cytoplasmic protein Ndr1 antibody
- DKFZp781G1938 antibody
see all
Images
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![Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)](https://www.abcam.com/ps/products/169/ab169775/Images/ab169775-168444-anti-ndrg2-antibody-epr11911b-western-blot.jpg)
Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)All lanes : Anti-NDRG2 antibody [EPR11911(B)] (ab169775) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HepG2 lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)](https://www.abcam.com/ps/products/169/ab169775/Images/ab169775-168443-anti-ndrg2-antibody-epr11911b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDRG2 with ab169775 at a 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)](https://www.abcam.com/ps/products/169/ab169775/Images/ab169775-168442-anti-ndrg2-antibody-epr11911b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling NDRG2 with ab169775 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Lee JC et al. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27589737) »
- Tsui KH et al. Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells. Sci Rep 5:12870 (2015). WB . Read more (PubMed: 26249737) »
