RecombinantRabMAb

Recombinant Anti-NDRG2 antibody [EPR11911(B)] (ab169775)

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Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
  • Anti-NDRG2 antibody [EPR11911(B)] (ab169775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11911(B)] to NDRG2
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NDRG2 antibody [EPR11911(B)]
    See all NDRG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11911(B)] to NDRG2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Orangutan, Xenopus tropicalis

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human NDRG2 (UniProt: Q9UN36).

  • Positive control

    • Human fetal heart, fetal brain, HepG2 or fetal kidney lysate, Human heart or liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.

    • Tissue specificity

      Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).

    • Sequence similarities

      Belongs to the NDRG family.

    • Developmental stage

      Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.

    • Cellular localization

      Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Antidepressant related protein ADRG123 antibody
      • Cytoplasmic protein Ndr1 antibody
      • DKFZp781G1938 antibody
      • FLJ25522 antibody
      • KIAA1248 antibody
      • N myc downstream regulated gene 2 antibody
      • N myc downstream regulator 2 antibody
      • NDR1 related protein NDR2 antibody
      • NDRG 2 antibody
      • NDRG family member 2 antibody
      • NDRG1 related protein antibody
      • NDRG2 antibody
      • NDRG2_HUMAN antibody
      • Protein NDRG2 antibody
      • Protein Syld709613 antibody
      • SYLD antibody
      • Syld709613 protein antibody
      see all

    Images

    • Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
      Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
      All lanes : Anti-NDRG2 antibody [EPR11911(B)] (ab169775) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)

      Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDRG2 with ab169775 at a 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (ab169775)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling NDRG2 with ab169775 at a 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-NDRG2 antibody [EPR11911(B)] (ab169775)
      Anti-NDRG2 antibody [EPR11911(B)] (ab169775)

    References (4)

    Publishing research using ab169775? Please let us know so that we can cite the reference in this datasheet.

    ab169775 has been referenced in 4 publications.

    • Xu T  et al. ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma. Aging (Albany NY) 12:1808-1827 (2020). PubMed: 32003757
    • Makieva S  et al. The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes. Am J Obstet Gynecol 216:602.e1-602.e21 (2017). PubMed: 28209491
    • Lee JC  et al. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo. Int J Mol Sci 17:N/A (2016). PubMed: 27589737
    • Tsui KH  et al. Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells. Sci Rep 5:12870 (2015). WB . PubMed: 26249737

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