Recombinant Anti-NDRG3 antibody [EPR9010(B)] (ab131266)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9010(B)] to NDRG3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDRG3 antibody [EPR9010(B)]
See all NDRG3 primary antibodies -
Description
Rabbit monoclonal [EPR9010(B)] to NDRG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji, Human fetal brain and LNCaP lysates; Human kidney and brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.92 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9010(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Ubiquitous. Highly expressed in brain. -
Sequence similarities
Belongs to the NDRG family. - Information by UniProt
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Database links
- Entrez Gene: 57446 Human
- Entrez Gene: 29812 Mouse
- Entrez Gene: 296318 Rat
- Omim: 605273 Human
- SwissProt: Q9UGV2 Human
- SwissProt: Q9QYF9 Mouse
- SwissProt: Q6AYR2 Rat
- Unigene: 437338 Human
see all -
Alternative names
- FLJ13556 antibody
- N myc downstream regulated gene 3 antibody
- N-myc downstream-regulated gene 3 protein antibody
see all
Images
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All lanes : Anti-NDRG3 antibody [EPR9010(B)] (ab131266) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling NDRG3 with ab131266 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human brain tissue labelling NDRG3 with ab131266 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab131266 has been referenced in 3 publications.
- Luo Z et al. LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Exp Ther Med 24:622 (2022). PubMed: 36160904
- Lee JC et al. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo. Int J Mol Sci 17:N/A (2016). PubMed: 27589737
- Tsui KH et al. Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells. Sci Rep 5:12870 (2015). WB . PubMed: 26249737