Overview

  • Product name

    Anti-NDRG3 antibody [EPR9011(B)]
    See all NDRG3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9011(B)] to NDRG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human NDRG3 (Q9UGV2).

  • Positive control

    • Human fetal brain lysate Raji cells and cell lysate, LnCaP cell lysate, Human kidney tissue, Human brain tissue. Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9011(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Ubiquitous. Highly expressed in brain.
    • Sequence similarities

      Belongs to the NDRG family.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ13556 antibody
      • N myc downstream regulated gene 3 antibody
      • N-myc downstream-regulated gene 3 protein antibody
      • NDRG family member 3 antibody
      • NDRG3 antibody
      • NDRG3_HUMAN antibody
      • Protein Ndr3 antibody
      • Protein NDRG3 antibody
      see all

    Images

    • All lanes : Anti-NDRG3 antibody [EPR9011(B)] (ab133715) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Raji cell lysate
      Lane 3 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 41 kDa

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling NDRG3 with ab133715 at 1/100.
    • Immunohistochemical analysis of paraffin embedded Human brain tissue labelling NDRG3 with ab133715 at 1/100.
    • Immunofluorescent staining of Raji cells labelling NDRG3 with ab133715 at 1/100.

    References

    This product has been referenced in:

    • Müller AK  et al. Proteomic Characterization of Prostate Cancer to Distinguish Nonmetastasizing and Metastasizing Primary Tumors and Lymph Node Metastases. Neoplasia 20:140-151 (2018). Read more (PubMed: 29248718) »
    • Zhang L  et al. Rab27b Is a Potential Indicator for Lymph Node Metastasis and Unfavorable Prognosis in Lung Adenocarcinoma. Dis Markers 2018:7293962 (2018). Read more (PubMed: 30627227) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133715.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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