Anti-NDRG4 antibody (ab224099)
Key features and details
- Rabbit polyclonal to NDRG4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDRG4 antibody
See all NDRG4 primary antibodies -
Description
Rabbit polyclonal to NDRG4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human NDRG4 aa 150-300.
Database link: Q9ULP0 -
Positive control
- WB: HEK-293T overexpressing NDRG4 cell lysate. IHC-P: Human cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37,38,39,40,43 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37,38,39,40,43 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the early postnatal development and function of neuronal cells. -
Tissue specificity
Expressed only in brain and heart. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain. -
Sequence similarities
Belongs to the NDRG family. - Information by UniProt
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Database links
- Entrez Gene: 65009 Human
- Entrez Gene: 234593 Mouse
- Entrez Gene: 64457 Rat
- Omim: 614463 Human
- SwissProt: Q9ULP0 Human
- SwissProt: Q8BTG7 Mouse
- SwissProt: Q9Z2L9 Rat
- Unigene: 322430 Human
see all -
Alternative names
- BDM1 antibody
- Brain development-related molecule 1 antibody
- KIAA1180 antibody
see all
Images
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All lanes : Anti-NDRG4 antibody (ab224099) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing NDRG4 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Predicted band size: 37,38,39,40,43 kDa -
Paraffin-embedded human cerebellum tissue stained for NDRG4 using ab224099 at 1/50 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224099 has not yet been referenced specifically in any publications.