Key features and details
- Rabbit polyclonal to NDRG4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NDRG4 antibody
See all NDRG4 primary antibodies
DescriptionRabbit polyclonal to NDRG4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: HEK-293T overexpressing NDRG4 cell lysate. IHC-P: Human cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 37,38,39,40,43 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in the early postnatal development and function of neuronal cells.
Tissue specificityExpressed only in brain and heart. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain.
Sequence similaritiesBelongs to the NDRG family.
- Information by UniProt
- BDM1 antibody
- Brain development-related molecule 1 antibody
- KIAA1180 antibody
All lanes : Anti-NDRG4 antibody (ab224099) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing NDRG4 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Predicted band size: 37,38,39,40,43 kDa
Paraffin-embedded human cerebellum tissue stained for NDRG4 using ab224099 at 1/50 dilution in immunohistochemical analysis.
ab224099 has not yet been referenced specifically in any publications.