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    nduf3-antibody-ab122270.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-NDUF3 antibody (ab122270)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
  • Western blot - Anti-NDUF3 antibody (ab122270)

Key features and details

  • Rabbit polyclonal to NDUF3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-NDUF3 antibody
  • Description

    Rabbit polyclonal to NDUF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    LLEPRIEIVV VGTGDRTERL QSQVLQAMRQ RGIAVEVQDT PNACATFNFL CHEGRVTGAA LIPPPGGTSL TSLGQAAQ, corresponding to amino acids 107-184 of Human NDUF3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: RT-4, U-251 MG, Human liver and tonsil lysate. IHC: Human kidney, prostate and testis tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • Involvement in disease

    Defects in NDUFAF3 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Cellular localization

    Nucleus. Mitochondrion inner membrane.
  • Target information above from: UniProt accession Q9BU61 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25915 Human
    • Omim: 612911 Human
    • SwissProt: Q9BU61 Human
    • Unigene: 31387 Human
    • Alternative names

      • C3orf60 antibody
      • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 antibody
      • NDUF3_HUMAN antibody
      • ndufaf3 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)

      ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)

      ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)

      ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.

    • Western blot - Anti-NDUF3 antibody (ab122270)
      Western blot - Anti-NDUF3 antibody (ab122270)
      All lanes : Anti-NDUF3 antibody (ab122270) at 1/250 dilution

      Lane 1 : RT-4 lysate
      Lane 2 : U251 -MG lysate
      Lane 3 : Human plasma lysate
      Lane 4 : Human liver lysate
      Lane 5 : Human tonsil lysate

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab122270? Please let us know so that we can cite the reference in this datasheet.

    ab122270 has not yet been referenced specifically in any publications.

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