Anti-NDUF3 antibody (ab122270)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NDUF3 antibody -
Description
Rabbit polyclonal to NDUF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
LLEPRIEIVV VGTGDRTERL QSQVLQAMRQ RGIAVEVQDT PNACATFNFL CHEGRVTGAA LIPPPGGTSL TSLGQAAQ, corresponding to amino acids 107-184 of Human NDUF3
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Positive control
- RT-4, U-251 MG, Human liver and tonsil lysate. Human duodenum tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250 - 1/500. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). -
Involvement in disease
Defects in NDUFAF3 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Cellular localization
Nucleus. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 25915 Human
- Entrez Gene: 56769 Rat
- Omim: 612911 Human
- SwissProt: Q9BU61 Human
- SwissProt: O08776 Rat
- Unigene: 31387 Human
- Unigene: 40118 Rat
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Alternative names
- C3orf60 antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 antibody
- NDUF3_HUMAN antibody
- ndufaf3 antibody
Images
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Western blot - Anti-NDUF3 antibody (ab122270)All lanes : Anti-NDUF3 antibody (ab122270) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U251 -MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Datasheets and documents
References
ab122270 has not yet been referenced specifically in any publications.
