Anti-NDUF3 antibody (ab122270)
Key features and details
- Rabbit polyclonal to NDUF3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDUF3 antibody -
Description
Rabbit polyclonal to NDUF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
LLEPRIEIVV VGTGDRTERL QSQVLQAMRQ RGIAVEVQDT PNACATFNFL CHEGRVTGAA LIPPPGGTSL TSLGQAAQ, corresponding to amino acids 107-184 of Human NDUF3
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Positive control
- WB: RT-4, U-251 MG, Human liver and tonsil lysate. IHC: Human kidney, prostate and testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). -
Involvement in disease
Defects in NDUFAF3 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Cellular localization
Nucleus. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 25915 Human
- Omim: 612911 Human
- SwissProt: Q9BU61 Human
- Unigene: 31387 Human
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Alternative names
- C3orf60 antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 antibody
- NDUF3_HUMAN antibody
- ndufaf3 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)
ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (ab122270)ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
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Western blot - Anti-NDUF3 antibody (ab122270)All lanes : Anti-NDUF3 antibody (ab122270) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U251 -MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122270 has not yet been referenced specifically in any publications.