Key features and details
- Rabbit polyclonal to NDUFA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NDUFA1 antibody
See all NDUFA1 primary antibodies
DescriptionRabbit polyclonal to NDUFA1
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a sequence at the C terminal of Human NDUFA1, different from the related rat and mouse sequences by one amino acid.
- Rat Heart, Brain, Liver and Kidney tissue lysates; HT1080 cell lysate; Rat Cardiac Muscle tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab131423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 8 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Tissue specificityPrimarily expressed in heart and skeletal muscle.
Involvement in diseaseDefects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I NDUFA1 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
All lanes : Anti-NDUFA1 antibody (ab131423) at 0.5 µg/ml
Lane 1 : Rat Heart tissue lysate
Lane 2 : Rat Brain tissue lysate
Lane 3 : Rat Liver tissue lysate
Lane 4 : Rat Kidney tissue lysate
Lane 5 : HT1080 Cell Lysate
Predicted band size: 8 kDa
Immunohistochemical analysis of paraffin-embedded Rat Cardiac Muscle Tissue labelling NDUFA1 with ab131423 at 1µg/ml.
ab131423 has not yet been referenced specifically in any publications.