Overview

  • Product name
    Anti-NDUFA1 antibody [EPR12083]
    See all NDUFA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12083] to NDUFA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
    Predicted to work with: GorillaDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA1 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O15239

  • Positive control
    • A673 cell lysate; Human skeletal muscle, Human fetal kidney and fetal heart lysates; Human heart and skeletal muscle tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 8 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • Tissue specificity
      Primarily expressed in heart and skeletal muscle.
    • Involvement in disease
      Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    • Sequence similarities
      Belongs to the complex I NDUFA1 subunit family.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CI MWFE antibody
      • CI-MWFE antibody
      • Complex I MWFE antibody
      • complex I MWFE subunit antibody
      • Complex I-MWFE antibody
      • MWFE antibody
      • MWFE, B. taurus, homolog of antibody
      • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 7.5kDa antibody
      • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 antibody
      • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 antibody
      • NADH oxidoreductase subunit MWFE antibody
      • NADH ubiquinone oxidoreductase (complex 1) antibody
      • NADH ubiquinone oxidoreductase MWFE subunit antibody
      • NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 antibody
      • NADH-ubiquinone oxidoreductase MWFE subunit antibody
      • NDUA1_HUMAN antibody
      • NDUFA 1 antibody
      • NDUFA1 antibody
      • Type I dehydrogenase antibody
      • ZNF 183 antibody
      • ZNF183 antibody
      see all

    Images

    • All lanes : Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human skeletal muscle lysate
      Lane 4 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 8 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.

    References

    ab176563 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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