Recombinant Anti-NDUFA1 antibody [EPR12083] (ab176563)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12083] to NDUFA1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NDUFA1 antibody [EPR12083]
See all NDUFA1 primary antibodies -
Description
Rabbit monoclonal [EPR12083] to NDUFA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: GorillaDoes not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- A673 cell lysate; Human skeletal muscle, Human fetal kidney and fetal heart lysates; Human heart and skeletal muscle tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12083 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 8 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
1/10 - 1/100.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 8 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
-
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Tissue specificity
Primarily expressed in heart and skeletal muscle. -
Involvement in disease
Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I NDUFA1 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 101144612 Gorilla
- Entrez Gene: 4694 Human
- Omim: 300078 Human
- SwissProt: Q8SPF0 Gorilla
- SwissProt: O15239 Human
- Unigene: 534168 Human
-
Alternative names
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
see all
Images
-
All lanes : Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 8 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab176563 has been referenced in 2 publications.
- Sen T et al. Decreased PGC1ß expression results in disrupted human erythroid differentiation, impaired hemoglobinization and cell cycle exit. Sci Rep 11:17129 (2021). PubMed: 34429458
- Jing YY et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978