Product nameAnti-NDUFA1 antibody [EPR12083]
See all NDUFA1 primary antibodies
DescriptionRabbit monoclonal [EPR12083] to NDUFA1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Predicted to work with: GorillaDoes not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA1 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O15239
- A673 cell lysate; Human skeletal muscle, Human fetal kidney and fetal heart lysates; Human heart and skeletal muscle tissues.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab176563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 8 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Tissue specificityPrimarily expressed in heart and skeletal muscle.
Involvement in diseaseDefects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I NDUFA1 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
All lanes : Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human skeletal muscle lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 8 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.
ab176563 has not yet been referenced specifically in any publications.