Recombinant Anti-NDUFA10 antibody [EPR12135] (ab174829)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-NDUFA10 antibody [EPR12135]
See all NDUFA10 primary antibodies -
Description
Rabbit monoclonal [EPR12135] to NDUFA10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA10 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O95299 -
Positive control
- HepG2, HeLa, Jurkat, Raji and Human fetal heart lysates; Human heart and kidney tissues; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12135 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa). | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. -
Cellular localization
Mitochondrion matrix. -
Database links
- Entrez Gene: 4705 Human
- Omim: 603835 Human
- SwissProt: O95299 Human
- Unigene: 277677 Human
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Alternative names
- CI 42KD antibody
- CI-42k antibody
- Complex I-42kD antibody
see all
Images
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![Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)](https://www.abcam.com/ps/products/174/ab174829/Images/ab174829-200285-anti-ndufa10-antibody-epr12135-western-blot.jpg)
Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)](https://www.abcam.com/ps/products/174/ab174829/Images/ab174829-200284-anti-ndufa10-antibody-epr12135-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)](https://www.abcam.com/ps/products/174/ab174829/Images/ab174829-200283-anti-ndufa10-antibody-epr12135-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)](https://www.abcam.com/ps/products/174/ab174829/Images/ab174829-200282-anti-ndufa10-antibody-epr12135-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)](https://www.abcam.com/ps/products/174/ab174829/Images/ab174829-200281-ab174829IP.jpg)
Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Basit F et al. Human Dendritic Cell Subsets Undergo Distinct Metabolic Reprogramming for Immune Response. Front Immunol 9:2489 (2018). Read more (PubMed: 30455688) »
- Khorsandi SE et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). Read more (PubMed: 29096719) »
