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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA10 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O95299
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab174829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"