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  1. Link

    ndufa10-antibody-epr12135-ab174829.pdf

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Signal Transduction Metabolism Mitochondrial
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Recombinant
RabMAb

Recombinant Anti-NDUFA10 antibody [EPR12135] (ab174829)

  • Datasheet
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  • Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)
  • Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)
  • Datasheet
  • References (2)
  • Protocols

Overview

  • Product name

    Anti-NDUFA10 antibody [EPR12135]
    See all NDUFA10 primary antibodies
  • Description

    Rabbit monoclonal [EPR12135] to NDUFA10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA10 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O95299

  • Positive control

    • HepG2, HeLa, Jurkat, Raji and Human fetal heart lysates; Human heart and kidney tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12135
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Associated products

  • Alternative Versions

    • Anti-NDUFA10 antibody [EPR12135] - BSA and Azide free (ab249821)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
    • HepG2 whole cell lysate (ab7900)

Applications

Our Abpromise guarantee covers the use of ab174829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      NDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
    • Cellular localization

      Mitochondrion matrix.
    • Database links

      • Entrez Gene: 4705 Human
      • Omim: 603835 Human
      • SwissProt: O95299 Human
      • Unigene: 277677 Human
      • Alternative names

        • CI 42KD antibody
        • CI-42k antibody
        • Complex I-42kD antibody
        • MGC5103 antibody
        • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 antibody
        • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa antibody
        • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody
        • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial complex I 42kDa subunit antibody
        • NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor antibody
        • NADH-ubiquinone oxidoreductase 42 kDa subunit antibody
        • ndufa10l antibody
        see all

      Images

      • Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : Raji cell lysate
        Lane 5 : Human fetal heart lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 41 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).

      • Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      Protocols

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Datasheets and documents

      • Datasheet
    • References

      This product has been referenced in:

      • Basit F  et al. Human Dendritic Cell Subsets Undergo Distinct Metabolic Reprogramming for Immune Response. Front Immunol 9:2489 (2018). Read more (PubMed: 30455688) »
      • Khorsandi SE  et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). Read more (PubMed: 29096719) »
      See all 2 Publications for this product

      Publishing research using ab174829? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab174829.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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