Overview

  • Product name
    Anti-NDUFA10 antibody [EPR12135]
    See all NDUFA10 primary antibodies
  • Description
    Rabbit monoclonal [EPR12135] to NDUFA10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA10 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O95299

  • Positive control
    • HepG2, HeLa, Jurkat, Raji and Human fetal heart lysates; Human heart and kidney tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Relevance
      NDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
    • Cellular localization
      Mitochondrion matrix.
    • Database links
      • Alternative names
        • CI 42KD antibody
        • CI-42k antibody
        • Complex I-42kD antibody
        • MGC5103 antibody
        • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 antibody
        • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa antibody
        • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody
        • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial complex I 42kDa subunit antibody
        • NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor antibody
        • NADH-ubiquinone oxidoreductase 42 kDa subunit antibody
        • ndufa10l antibody
        see all

      Images

      • Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Western blot - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : Raji cell lysate
        Lane 5 : Human fetal heart lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 41 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.

      • Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunocytochemistry/ Immunofluorescence - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).

      • Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)
        Immunoprecipitation - Anti-NDUFA10 antibody [EPR12135] (ab174829)

        Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      References

      This product has been referenced in:
      • Khorsandi SE  et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). Read more (PubMed: 29096719) »

      See 1 Publication for this product

      Publishing research using ab174829? Please let us know so that we can cite the reference in this datasheet.

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