Product nameAnti-NDUFA10 antibody [EPR12135]
See all NDUFA10 primary antibodies
DescriptionRabbit monoclonal [EPR12135] to NDUFA10
Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA10 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O95299
- HepG2, HeLa, Jurkat, Raji and Human fetal heart lysates; Human heart and kidney tissues; HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab174829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceNDUFA10 protein belongs to the complex I 42kDa subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
Cellular localizationMitochondrion matrix.
- CI 42KD antibody
- CI-42k antibody
- Complex I-42kD antibody
All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This product has been referenced in:
- Basit F et al. Human Dendritic Cell Subsets Undergo Distinct Metabolic Reprogramming for Immune Response. Front Immunol 9:2489 (2018). Read more (PubMed: 30455688) »
- Khorsandi SE et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). Read more (PubMed: 29096719) »