Anti-NDUFA2 antibody (ab198196)
Key features and details
- Rabbit polyclonal to NDUFA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFA2 antibody -
Description
Rabbit polyclonal to NDUFA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Cynomolgus monkey, Gorilla, Orangutan -
Immunogen
Recombinant full length protein (GST-tag) corresponding to Human NDUFA2 aa 1 to the C-terminus.
Database link: O43678 -
Positive control
- IHC: Human esophagus and breast cancer tissue; WB: Mouse heart tissue lysate,
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General notes
The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 11 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 11 kDa. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 327698 Cow
- Entrez Gene: 101925194 Cynomolgus monkey
- Entrez Gene: 101147705 Gorilla
- Entrez Gene: 4695 Human
- Entrez Gene: 17991 Mouse
- Entrez Gene: 100173647 Orangutan
- Omim: 602137 Human
- SwissProt: Q02370 Cow
see all -
Alternative names
- B8 antibody
- CI B8 antibody
- CI-B8 antibody
see all
Images
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Anti-NDUFA2 antibody (ab198196) at 1/400 dilution + Mouse heart tissue lysate at 40 µg
Secondary
Goat anti rabbit IgG at 1/5000 dilution
Predicted band size: 11 kDa
Exposure time: 2 minutesGel: 12%SDS-PAGE.
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Immunohischemical analysis of paraffin-embedded Human breast cancer tissue using ab198196 at dilution 1/60.
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Immunohischemical analysis of paraffin-embedded Human esophagus cancer tissue using ab198196 at dilution 1/60.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab198196 has been referenced in 1 publication.
- Wang Z et al. High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma. Transl Oncol 14:101146 (2021). PubMed: 34118692