Overview

  • Product name

    Anti-NDUFA4 antibody
  • Description

    Rabbit polyclonal to NDUFA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of Human NDUFA4.

  • Positive control

    • HepG2 and RAW264.7 cell extracts This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab129752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 9 kDa.
ELISA 1/10000.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFA4 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CI 9k antibody
    • CI-MLRQ antibody
    • Complex I 9kDa subunit antibody
    • Complex I-MLRQ antibody
    • MLRQ antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 9kD antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 antibody
    • NADH-ubiquinone oxidoreductase MLRQ subunit antibody
    • NDUA4_HUMAN antibody
    • NDUFA4 antibody
    see all

Images

  • ab129752 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129752 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-NDUFA4 antibody (ab129752) at 1/100 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunizing peptide
    Lane 3 : RAW264.7 cell extract

    Predicted band size: 9 kDa

References

This product has been referenced in:

  • Cui S  et al. LncRNA MAFG-AS1 promotes the progression of colorectal cancer by sponging miR-147b and activation of NDUFA4. Biochem Biophys Res Commun 506:251-258 (2018). Read more (PubMed: 30348529) »
  • Lee D  et al. Folate cycle enzyme MTHFD1L confers metabolic advantages in hepatocellular carcinoma. J Clin Invest 127:1856-1872 (2017). Read more (PubMed: 28394261) »
See all 2 Publications for this product

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