Product nameAnti-NDUFA4 antibody
DescriptionRabbit polyclonal to NDUFA4
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide derived from internal sequence of Human NDUFA4.
- HepG2 and RAW264.7 cell extracts This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab129752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 9 kDa.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFA4 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI 9k antibody
- CI-MLRQ antibody
- Complex I 9kDa subunit antibody
ab129752 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129752 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-NDUFA4 antibody (ab129752) at 1/100 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunizing peptide
Lane 3 : RAW264.7 cell extract
Predicted band size: 9 kDa
This product has been referenced in:
- Cui S et al. LncRNA MAFG-AS1 promotes the progression of colorectal cancer by sponging miR-147b and activation of NDUFA4. Biochem Biophys Res Commun 506:251-258 (2018). Read more (PubMed: 30348529) »
- Lee D et al. Folate cycle enzyme MTHFD1L confers metabolic advantages in hepatocellular carcinoma. J Clin Invest 127:1856-1872 (2017). Read more (PubMed: 28394261) »