Overview

  • Product name

  • Description

    Rabbit polyclonal to NDUFA4L2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human NDUFA4L2.

  • Positive control

    • Extracts from Jurkat cells, COLO cells, HUVEC cells and HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
ELISA 1/20000.

Target

Images

  • All lanes : Anti-NDUFA4L2 antibody (ab74138) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HepG2 cells
    Lane 5 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 10 kDa
    Observed band size: <19 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zheng J  et al. Induction of the mitochondrial NDUFA4L2 protein by HIF-1a regulates heart regeneration by promoting the survival of cardiac stem cell. Biochem Biophys Res Commun N/A:N/A (2018). WB ; Rat . Read more (PubMed: 29953852) »
  • Miller BA  et al. TRPM2 channels protect against cardiac ischemia-reperfusion injury: role of mitochondria. J Biol Chem 289:7615-29 (2014). WB ; Mouse . Read more (PubMed: 24492610) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse adrenal gland)
Permeabilization
Yes - 0.1% v/v Tween 20
Specification
Mouse adrenal gland
Fixative
Paraformaldehyde

Maria Eleni Kastriti

Verified customer

Submitted Jan 17 2018

Application
Western blot
Sample
Rat Tissue lysate - other (liver)
Gel Running Conditions
Reduced Denaturing (any kd)
Loading amount
75 µg
Treatment
detergent
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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