Overview

  • Product name

    Anti-NDUFA5 antibody [EPR15330]
    See all NDUFA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR15330] to NDUFA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein within Human NDUFA5 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16718

  • Positive control

    • Human fetal heart, HepG2, 293 and HT-1376 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Tissue specificity

    Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.
  • Sequence similarities

    Belongs to the complex I NDUFA5 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B13 antibody
    • CI 13kB antibody
    • CI-13kD-B antibody
    • Complex I subunit B13 antibody
    • Complex I-13kD-B antibody
    • DKFZp781K1356 antibody
    • FLJ12147 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 antibody
    • NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody
    • NDUA5_HUMAN antibody
    • NDUFA5 antibody
    • NUFM antibody
    • Type I dehydrogenase antibody
    • Ubiquinone reductase antibody
    • UQOR13 antibody
    see all

Images

  • All lanes : Anti-NDUFA5 antibody [EPR15330] (ab183706) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HT-1376 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • Anti-NDUFA5 antibody [EPR15330] (ab183706) at 1/20000 dilution + Human fetal heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

References

This product has been referenced in:

  • Tan EP  et al. Altering O-linked ß-N-acetylglucosamine cycling disrupts mitochondrial function. J Biol Chem 289:14719-30 (2014). WB . Read more (PubMed: 24713701) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HEK293
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Neutral buffered formalin

Abcam user community

Verified customer

Submitted May 17 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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