Overview

  • Product name

  • Description

    Goat polyclonal to NDUFA7
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human NDUFA7 aa 39-62 (internal sequence) (Cysteine residue).
    Sequence:

    PKLPVGPSHKLSNN


    Database link: NP_004992.2

  • Positive control

    • WB: Human, mouse, pig and rat heart lysates. IHC-P: Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab140871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue sections labelling NDUFA7 with ab140871 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • All lanes : Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Mouse heart lysate (in RIPA buffer)
    Lane 3 : Rat heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

This product has been referenced in:

  • Zhu Z  et al. Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm. Front Physiol 10:252 (2019). Read more (PubMed: 30914972) »
See 1 Publication for this product

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