Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
20 µg
Specification
HEK 293T
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% PBST

Secondary antibody

Name
Non-Abcam antibody was used: Upstate Goat Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 39 kDa

Mr. Brian Koss

Verified customer

Submitted Dec 01 2011

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