Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Excellent
Abreviews
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
fibroblasts, mitochondrial fraction
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 23°C · Diluent: PBS 1% BSA
Concentration
1 µg/ml

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Cell Signaling Technology Anti-mouse IgG #7076
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
SuperSignal Pico ECL
Negative control
Turdis migratorius fibroblasts, cytoplasmic fraction
Exposure
10 second(s)
Bands
Specific: 37 kDa
Positive control
AML12 hepatocytes, mitochondrial fraction

Additional data

Additional Notes
Mitochondrial and cytoplasmic fractions were obtained from robin fibroblast and mouse hepatocyte cell lines with Abcam Mitochondrial Isolation Kit for Cells (ab110170).

Abcam user community

Verified customer

Submitted Sep 07 2017

Sign up