Western blot abreview for Anti-NDUFA9 antibody [20C11B11B11]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (Mitochondrial extract)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mitochondrial extract
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Positive control
observed 36KDa band in control sample

Additional data

PubMed ID
27828948: View

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Submitted Feb 07 2018

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