Product nameAnti-NDUFA9 antibody
See all NDUFA9 primary antibodies
DescriptionRabbit polyclonal to NDUFA9
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rabbit, Horse, Dog, Human, Chimpanzee, Gorilla, Chinese hamster, Orangutan
- This antibody gave a positive signal in both Mouse Testis and Heart tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab128744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFA9 subunit family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- CI-39kD antibody
- Complex I subunit NDUFA9 antibody
- Complex I-39kD antibody
All lanes : Anti-NDUFA9 antibody (ab128744) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 35 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128744 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab128744 has not yet been referenced specifically in any publications.