Anti-NDUFA9 antibody (ab128744)

Rabbit polyclonal NDUFA9 antibody. Validated in WB and tested in Mouse. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to NDUFA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rabbit, Horse, Dog, Human, Chimpanzee, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human NDUFA9 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q16795
    (Peptide available as ab153676)

  • Positive control

    • This antibody gave a positive signal in both Mouse Testis and Heart tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab128744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFA9 subunit family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CI-39kD antibody
    • Complex I subunit NDUFA9 antibody
    • Complex I-39kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial antibody
    • NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 antibody
    • NADH-ubiquinone oxidoreductase 39 kDa subunit antibody
    • NDUA9_HUMAN antibody
    • NDUFA9 antibody
    see all

Images

  • All lanes : Anti-NDUFA9 antibody (ab128744) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    The band observed at 35 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128744 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab128744 has not yet been referenced specifically in any publications.

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