Product nameAnti-NDUFA9 antibody
See all NDUFA9 primary antibodies
DescriptionRabbit polyclonal to NDUFA9
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- ICC/IF: RH-30 cells. WB: RT4 and U-251 MG cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab221940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/100 - 1/250. Predicted molecular weight: 43 kDa.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFA9 subunit family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- CI-39kD antibody
- Complex I subunit NDUFA9 antibody
- Complex I-39kD antibody
All lanes : Anti-NDUFA9 antibody (ab221940) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 43 kDa
PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for NDUFA9 (green) using ab221940 at 4 µg/ml in ICC/IF.
ab221940 has not yet been referenced specifically in any publications.