Recombinant Anti-NDUFAB1 antibody [EPR6725] (ab181021)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6725] to NDUFAB1
- Suitable for: Flow Cyt (Intra), IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NDUFAB1 antibody [EPR6725]
See all NDUFAB1 primary antibodies -
Description
Rabbit monoclonal [EPR6725] to NDUFAB1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Molt-4, A431 and HepG2 cell lysate. Human heart and kidney tissue. HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6725 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/160.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/60.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/60. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa. |
Target
-
Function
Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. -
Sequence similarities
Contains 1 acyl carrier domain. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 4706 Human
- Entrez Gene: 70316 Mouse
- Omim: 603836 Human
- SwissProt: O14561 Human
- SwissProt: Q9CR21 Mouse
- Unigene: 189716 Human
- Unigene: 3014 Mouse
- Unigene: 347976 Mouse
-
Alternative names
- ACP antibody
- ACPM_HUMAN antibody
- Acyl carrier protein antibody
see all
Images
-
All lanes : Anti-NDUFAB1 antibody [EPR6725] (ab181021) at 1/10000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
-
Immunohistochemical analysis of paraffin embedded Human heart tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Intracellular Flow Cytometry analysis of HepG2 cells labeling NDUFAB1 using ab181021 at a 1/160 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
-
Lysate from HepG2 cells was immunoprecipitated with ab181021 at a 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab181021 has been referenced in 3 publications.
- Fu Q et al. Proteomics reveals MRPL4 as a high-risk factor and a potential diagnostic biomarker for prostate cancer. Proteomics 22:e2200081 (2022). PubMed: 36059095
- Jing YY et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978
- Majmudar JD et al. 4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function. Medchemcomm 10:209-220 (2019). PubMed: 30881609