Recombinant Anti-NDUFAF1 antibody [EPR2795] (ab92454)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2795] to NDUFAF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDUFAF1 antibody [EPR2795]
See all NDUFAF1 primary antibodies -
Description
Rabbit monoclonal [EPR2795] to NDUFAF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NDUFAF1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2795 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CIA30 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 51103 Human
- Omim: 606934 Human
- SwissProt: Q9Y375 Human
- Unigene: 106529 Human
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Alternative names
- CGI 65 antibody
- CGI65 antibody
- CIA30 antibody
see all
Images
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All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92454 has been referenced in 1 publication.
- Lindfors C et al. Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108:18108-13 (2011). WB . PubMed: 22025706