Overview

  • Product name

    Anti-NDUFAF4 antibody [EPR15931]
    See all NDUFAF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR15931] to NDUFAF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human NDUFAF4 aa 1 to the C-terminus.
    Database link: Q9P032

  • Positive control

    • T47D, Raji, K562, MCF7 and MDA-MB-435 cell lysates; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IP 1/90.
ICC/IF 1/150.

Target

  • Function

    Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
  • Involvement in disease

    Mitochondrial complex I deficiency
  • Sequence similarities

    Belongs to the NDUFAF4 family.
  • Post-translational
    modifications

    Phosphorylated on serine. Prolactin stimulate serine phosphorylation.
  • Cellular localization

    Mitochondrion. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C6orf66 antibody
    • Hormone-regulated proliferation-associated protein of 20 kDa antibody
    • HRPAP20 antibody
    • HSPC125 antibody
    • My013 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 antibody
    • NDUF4_HUMAN antibody
    • Ndufaf4 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.

  • All lanes : Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/10000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/1000 dilution + MCF7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of MDA-MB-435 cell lysate.

References

ab191414 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191414.
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