Recombinant Anti-NDUFAF4 antibody [EPR15931] (ab191414)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15931] to NDUFAF4
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDUFAF4 antibody [EPR15931]
See all NDUFAF4 primary antibodies -
Description
Rabbit monoclonal [EPR15931] to NDUFAF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47D, Raji, K562, MCF7 and MDA-MB-435 cell lysates; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15931 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IP |
1/90.
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ICC/IF |
1/150.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IP
1/90. |
ICC/IF
1/150. |
Target
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Function
Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. -
Involvement in disease
Mitochondrial complex I deficiency -
Sequence similarities
Belongs to the NDUFAF4 family. -
Post-translational
modificationsPhosphorylated on serine. Prolactin stimulate serine phosphorylation. -
Cellular localization
Mitochondrion. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 29078 Human
- Omim: 252010 Human
- SwissProt: Q9P032 Human
- Unigene: 512144 Human
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Alternative names
- C6orf66 antibody
- Hormone-regulated proliferation-associated protein of 20 kDa antibody
- HRPAP20 antibody
see all
Images
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Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.
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All lanes : Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/10000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : Raji cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/1000 dilution + MCF7 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of MDA-MB-435 cell lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191414 has not yet been referenced specifically in any publications.