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  1. Link

    ndufb10-antibody-epr16230-47-ab196019.pdf

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Signal Transduction Metabolism Energy Metabolism
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Recombinant
RabMAb

Recombinant Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

  • Datasheet
Reviews (1) Submit a question References (3)

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  • Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
  • Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
  • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

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  • Datasheet
  • References (3)
  • Protocols

Overview

  • Product name

    Anti-NDUFB10 antibody [EPR16230-47]
    See all NDUFB10 primary antibodies
  • Description

    Rabbit monoclonal [EPR16230-47] to NDUFB10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NDUFB10 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O96000

  • Positive control

    • HeLa, HepG2 and Jurkat cell lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells; HeLa whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16230-47
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Associated products

  • Alternative Versions

    • Anti-NDUFB10 antibody [EPR16230-47] - BSA and Azide free (ab251216)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab196019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/350.
IP 1/50.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Flow Cyt 1/800.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFB10 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession O96000 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4716 Human
    • Entrez Gene: 68342 Mouse
    • Entrez Gene: 681418 Rat
    • Omim: 603843 Human
    • SwissProt: O96000 Human
    • SwissProt: Q9DCS9 Mouse
    • Unigene: 513266 Human
    • Unigene: 1129 Mouse
    • Alternative names

      • 0610011B04Rik antibody
      • 22kDa antibody
      • CI PDSW antibody
      • CI-PDSW antibody
      • Complex I PDSW antibody
      • Complex I PDSW subunit antibody
      • Complex I-PDSW antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 antibody
      • NADH ubiquinone oxidoreductase PDSW subunit antibody
      • NADH-ubiquinone oxidoreductase PDSW subunit antibody
      • NDUBA_HUMAN antibody
      • NDUFB 10 antibody
      • Ndufb10 antibody
      • PDSW antibody
      see all

    Images

    • Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      All lanes : Anti-NDUFB10 antibody [EPR16230-47] (ab196019) at 1/10000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

      Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NDUFB10 with ab196019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

      Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NDUFB10 with ab196019 at 1/350 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue).

    • Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB10 (red) with purified ab196019 at a dilution of 1/800. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

    • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
      Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

      NDUFB10 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196019 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196019 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196019 in HeLa whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Habich M  et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). Read more (PubMed: 30650365) »
    • Osuagwu N  et al. Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or a-synuclein, is localized to the mitochondrial matrix. PLoS One 14:e0219909 (2019). Read more (PubMed: 31323073) »
    See all 3 Publications for this product

    Publishing research using ab196019? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NDUFB10 antibody [EPR16230-47]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Neutral buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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