Recombinant Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-NDUFB10 antibody [EPR16230-47]
See all NDUFB10 primary antibodies -
Description
Rabbit monoclonal [EPR16230-47] to NDUFB10 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human NDUFB10 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: O96000 -
Positive control
- HeLa, HepG2 and Jurkat cell lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells; HeLa whole cell extract.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16230-47 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab196019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/350. | |
| IP | 1/50. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| Flow Cyt | 1/800. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFB10 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4716 Human
- Entrez Gene: 68342 Mouse
- Entrez Gene: 681418 Rat
- Omim: 603843 Human
- SwissProt: O96000 Human
- SwissProt: Q9DCS9 Mouse
- Unigene: 513266 Human
- Unigene: 1129 Mouse
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Alternative names
- 0610011B04Rik antibody
- 22kDa antibody
- CI PDSW antibody
see all
Images
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![Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)](https://www.abcam.com/ps/products/196/ab196019/Images/ab196019-239997-anti-ndufb10-antibody-epr16230-47-western-blot.jpg)
Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)All lanes : Anti-NDUFB10 antibody [EPR16230-47] (ab196019) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)](https://www.abcam.com/ps/products/196/ab196019/Images/ab196019-239999-anti-ndufb10-antibody-epr16230-47-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NDUFB10 with ab196019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.
Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)](https://www.abcam.com/ps/products/196/ab196019/Images/ab196019-253678-anti-ndufb10-antibody-epr16230-47-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NDUFB10 with ab196019 at 1/350 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue).
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![Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)](https://www.abcam.com/ps/products/196/ab196019/Images/ab196019-265189-anti-ndufb10-antibody-epr16230-47-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB10 (red) with purified ab196019 at a dilution of 1/800. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.
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![Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)](https://www.abcam.com/ps/products/196/ab196019/Images/ab196019-240001-anti-ndufb10-antibody-epr16230-47-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)NDUFB10 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196019 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196019 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196019 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Habich M et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). Read more (PubMed: 30650365) »
- Osuagwu N et al. Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or a-synuclein, is localized to the mitochondrial matrix. PLoS One 14:e0219909 (2019). Read more (PubMed: 31323073) »
