Recombinant Anti-NDUFB10 antibody [EPR16230] (ab192245)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16230] to NDUFB10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDUFB10 antibody [EPR16230]
See all NDUFB10 primary antibodies -
Description
Rabbit monoclonal [EPR16230] to NDUFB10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2, Jurkat and Human fetal kidney lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16230 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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ICC/IF |
1/50.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ICC/IF
1/50. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFB10 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4716 Human
- Omim: 603843 Human
- SwissProt: O96000 Human
- Unigene: 513266 Human
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Alternative names
- 0610011B04Rik antibody
- 22kDa antibody
- CI PDSW antibody
see all
Images
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All lanes : Anti-NDUFB10 antibody [EPR16230] (ab192245) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with ab192245 at 1/50 dilution ( 6 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).
The two negative controls are ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab192245 has been referenced in 1 publication.
- Wang C et al. MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex. EMBO Rep 21:e48833 (2020). PubMed: 31721420