Overview

  • Product name

    Anti-NDUFB10 antibody [EPR16230]
    See all NDUFB10 primary antibodies
  • Description

    Rabbit monoclonal [EPR16230] to NDUFB10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NDUFB10 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O96000

  • Positive control

    • HeLa, HepG2, Jurkat and Human fetal kidney lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF 1/50.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFB10 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610011B04Rik antibody
    • 22kDa antibody
    • CI PDSW antibody
    • CI-PDSW antibody
    • Complex I PDSW antibody
    • Complex I PDSW subunit antibody
    • Complex I-PDSW antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 antibody
    • NADH ubiquinone oxidoreductase PDSW subunit antibody
    • NADH-ubiquinone oxidoreductase PDSW subunit antibody
    • NDUBA_HUMAN antibody
    • NDUFB 10 antibody
    • Ndufb10 antibody
    • PDSW antibody
    see all

Images

  • All lanes : Anti-NDUFB10 antibody [EPR16230] (ab192245) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with ab192245 at 1/50 dilution ( 6 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

References

ab192245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192245.
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