RecombinantRabMAb

Recombinant Anti-NDUFB11 antibody [EPR15252] (ab183716)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15252] to NDUFB11
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFB11 antibody [EPR15252]
    See all NDUFB11 primary antibodies

  • Description

    Rabbit monoclonal [EPR15252] to NDUFB11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human NDUFB11 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NX14

  • Positive control

    • Human heart lysate; HeLa, HepG2 and A431 cell lysates; Human kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab183716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    NDUFB11 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11) is a component of the mitochondrial complex 1. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. Complex I transfers electrons from NADH to the respiratory chain via ubiquinone.

  • Cellular localization

    Mitochondrion inner membrane.

  • Database links

    • Alternative names

      • CI ESSS antibody
      • Complex I ESSS antibody
      • complex I NP17.3 subunit antibody
      • ESSS antibody
      • FLJ20494 antibody
      • MGC111182 antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 17.3kDa antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 mitochondrial antibody
      • NADH ubiquinone oxidoreductase ESSS subunit antibody
      • NDUFB 11 antibody
      • Neuronal protein 17.3 antibody
      • Np15 antibody
      • NP17.3 antibody
      • P17.3 antibody
      see all

    Images

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution + Human heart lysate at 20 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Lanes 1-2 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
      Lane 3 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

    • Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Anti-NDUFB11 antibody [EPR15252] (ab183716)

    References (3)

    Publishing research using ab183716? Please let us know so that we can cite the reference in this datasheet.

    ab183716 has been referenced in 3 publications.

    • Timón-Gómez A  et al. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep 31:107607 (2020). PubMed: 32375044
    • Baertling F  et al. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin Genet 93:111-118 (2018). WB ; Human . PubMed: 28671271
    • Reinson K  et al. Diverse phenotype in patients with complex I deficiency due to mutations in NDUFB11. Eur J Med Genet N/A:N/A (2018). PubMed: 30423443

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