Recombinant
RabMAb

Recombinant Anti-NDUFB11 antibody [EPR15252] (ab183716)

Submit a review Submit a question References (2)

Overview

  • Product name

    Anti-NDUFB11 antibody [EPR15252]
    See all NDUFB11 primary antibodies

  • Description

    Rabbit monoclonal [EPR15252] to NDUFB11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human NDUFB11 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NX14

  • Positive control

    • Human heart lysate; HeLa, HepG2 and A431 cell lysates; Human kidney tissue; HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    NDUFB11 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11) is a component of the mitochondrial complex 1. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. Complex I transfers electrons from NADH to the respiratory chain via ubiquinone.

  • Cellular localization

    Mitochondrion inner membrane.

  • Database links

    • Alternative names

      • CI ESSS antibody
      • Complex I ESSS antibody
      • complex I NP17.3 subunit antibody
      • ESSS antibody
      • FLJ20494 antibody
      • MGC111182 antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 17.3kDa antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 mitochondrial antibody
      • NADH ubiquinone oxidoreductase ESSS subunit antibody
      • NDUFB 11 antibody
      • Neuronal protein 17.3 antibody
      • Np15 antibody
      • NP17.3 antibody
      • P17.3 antibody
      see all

    Images

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution + Human heart lysate at 20 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Lanes 1-2 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
      Lane 3 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

    References

    This product has been referenced in:

    • Baertling F  et al. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin Genet 93:111-118 (2018). WB ; Human . Read more (PubMed: 28671271) »
    • Reinson K  et al. Diverse phenotype in patients with complex I deficiency due to mutations in NDUFB11. Eur J Med Genet N/A:N/A (2018). Read more (PubMed: 30423443) »
    See all 2 Publications for this product

    Publishing research using ab183716? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab183716.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com