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  1. Link

    ndufb11-antibody-epr15252-ab183716.pdf

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Recombinant
RabMAb

Recombinant Anti-NDUFB11 antibody [EPR15252] (ab183716)

  • Datasheet
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  • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
  • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
  • Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)

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  • Datasheet
  • References (2)
  • Protocols

Overview

  • Product name

    Anti-NDUFB11 antibody [EPR15252]
    See all NDUFB11 primary antibodies
  • Description

    Rabbit monoclonal [EPR15252] to NDUFB11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NDUFB11 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NX14

  • Positive control

    • Human heart lysate; HeLa, HepG2 and A431 cell lysates; Human kidney tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15252
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Associated products

  • Alternative Versions

    • Anti-NDUFB11 antibody [EPR15252] - BSA and Azide free (ab250690)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit (ab102918)
    • APC Conjugation Kit (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • HepG2 whole cell lysate (ab7900)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human NDUFB11 protein (ab162774)
  • Related Products

    • Recombinant Human NDUFB11 protein (ab162774)

Applications

Our Abpromise guarantee covers the use of ab183716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    NDUFB11 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11) is a component of the mitochondrial complex 1. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. Complex I transfers electrons from NADH to the respiratory chain via ubiquinone.
  • Cellular localization

    Mitochondrion inner membrane.
  • Database links

    • Entrez Gene: 54539 Human
    • Entrez Gene: 299310 Rat
    • Omim: 300403 Human
    • SwissProt: Q9NX14 Human
    • Unigene: 3377 Rat
    • Alternative names

      • CI ESSS antibody
      • Complex I ESSS antibody
      • complex I NP17.3 subunit antibody
      • ESSS antibody
      • FLJ20494 antibody
      • MGC111182 antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 17.3kDa antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 mitochondrial antibody
      • NADH ubiquinone oxidoreductase ESSS subunit antibody
      • NDUFB 11 antibody
      • Neuronal protein 17.3 antibody
      • Np15 antibody
      • NP17.3 antibody
      • P17.3 antibody
      see all

    Images

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution + Human heart lysate at 20 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Lanes 1-2 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
      Lane 3 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)
      Flow Cytometry - Anti-NDUFB11 antibody [EPR15252] (ab183716)

      Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Baertling F  et al. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin Genet 93:111-118 (2018). WB ; Human . Read more (PubMed: 28671271) »
    • Reinson K  et al. Diverse phenotype in patients with complex I deficiency due to mutations in NDUFB11. Eur J Med Genet N/A:N/A (2018). Read more (PubMed: 30423443) »
    See all 2 Publications for this product

    Publishing research using ab183716? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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