Recombinant
RabMAb

Recombinant Anti-NDUFB2 antibody [EPR15569] (ab186748)

Overview

  • Product name

    Anti-NDUFB2 antibody [EPR15569]
    See all NDUFB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15569] to NDUFB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NDUFB2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O95178

  • Positive control

    • Human skeletal muscle, fetal heart, fetal brain, fetal kidney, HeLa and 293 lysates; Rat kidney, heart and brain lysates; Mouse heart, kidney and spleen lysates; Human endometrium tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P 1/50 - 1/100.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFB2 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CI-AGGG antibody
    • Complex I-AGGG antibody
    • mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 antibody
    • NADH-ubiquinone oxidoreductase AGGG subunit antibody
    • NDUB2_HUMAN antibody
    • NDUFB2 antibody
    see all

Images

  • Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/10000 dilution + Human fetal heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa

  • All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFB2 with ab186748 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Flow Cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling NDUFB2 with ab186748 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab186748 has not yet been referenced specifically in any publications.

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