Overview

  • Product name
    Anti-NDUFB4 antibody [17G3D9E12]
    See all NDUFB4 primary antibodies
  • Description
    Mouse monoclonal [17G3D9E12] to NDUFB4
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    NDUFB4 from cow

  • Positive control
    • Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 5 - 15 µg/ml.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

Flow Cyt Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal).

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB4 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B15 antibody
    • CI-B15 antibody
    • Complex I B15 subunit antibody
    • Complex I-B15 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody
    • NADH ubiquinone oxidoreductase B15 subunit antibody
    • NADH-ubiquinone oxidoreductase B15 subunit antibody
    • NDUB4_HUMAN antibody
    • NDUFB4 antibody
    see all

Images

  • All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg

    Predicted band size: 15 kDa

  • Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
    Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.

  • HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.

References

This product has been referenced in:
  • Anand R  et al. Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells. PLoS One 11:e0160258 (2016). WB . Read more (PubMed: 27479602) »
  • Chang HY  et al. Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells. Cell Death Dis 5:e1540 (2014). IF ; Human . Read more (PubMed: 25429617) »
See all 12 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain - Cerebellum)
Specification
Brain - Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization
No

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

Answer

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

Complex I NDUFB4: ab110243
https://www.abcam.com/index.html?datasheet=110243 https://www.abcam.com/index.html?datasheet=110243.

Complex II-30: ab14714
https://www.abcam.com/index.html?datasheet=14714 https://www.abcam.com/index.html?datasheet=14714.

Complex III-Core 2: ab14745
https://www.abcam.com/index.html?datasheet=14745 https://www.abcam.com/index.html?datasheet=14745.

Complex IV-1: ab14705
https://www.abcam.com/index.html?datasheet=14705 https://www.abcam.com/index.html?datasheet=14705.

Complex V-OSCP: ab110276
https://www.abcam.com/index.html?datasheet=110276 https://www.abcam.com/index.html?datasheet=110276.

PDH-E1 alpha: ab110330
https://www.abcam.com/index.html?datasheet=110330 https://www.abcam.com/index.html?datasheet=110330.

Complex V alpha: ab14748
https://www.abcam.com/index.html?datasheet=14748 https://www.abcam.com/index.html?datasheet=14748.

I understand that it would be expensive to purchase all these together. However, if you purchase more than 5 items on one order, we are able to provide a discount. If you are interseted in this, please contact our customer service department (orders@abcam.com) with a complete list of the ones you wish to order, and they will be pleased to provide a quote for you.

I am sorry we do not have exactly what you require on this occasion, but I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that product is not available any longer, there is ab110411, Total OXPHOS Human WB Antibody Cocktail and ab110416, Pyruvate dehydrogenase (PDH) WB Antibody Cocktail, however as the name suggests they have only been tested in WB, not IHC-P.

If you would like to considering trying to recreate the original antibody cocktail,I have listed the individual components are below.

Complex I 17G3D9E12 ab110243
https://www.abcam.com/index.html?datasheet=110243 (or use the following: https://www.abcam.com/index.html?datasheet=110243).

Complex II ab14714
https://www.abcam.com/index.html?datasheet=14714 (or use the following: https://www.abcam.com/index.html?datasheet=14714).

Complex III 13G12AF12BB11 ab14745
https://www.abcam.com/index.html?datasheet=14745 (or use the following: https://www.abcam.com/index.html?datasheet=14745).

Complex IV 1D6E1A8 ab14705
https://www.abcam.com/index.html?datasheet=14705 (or use the following: https://www.abcam.com/index.html?datasheet=14705).

Complex V 4C11C10D12 ab110276
https://www.abcam.com/index.html?datasheet=110276 (or use the following: https://www.abcam.com/index.html?datasheet=110276).

PDH 9H9AF5 ab110330
https://www.abcam.com/index.html?datasheet=110330 (or use the following: https://www.abcam.com/index.html?datasheet=110330).

As there are several components, we would be able to offer you a discount if you wish to purchase all of these products together. I am forwarding this to our bulk orders team who will be able to provide a quote for you.

Otherwise, I am sorry there is nothing else to suggest that would be a suitable replacement on this occasion.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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