• Product name
    Anti-NDUFB4 antibody [17G3D9E12]
    See all NDUFB4 primary antibodies
  • Description
    Mouse monoclonal [17G3D9E12] to NDUFB4
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    NDUFB4 from cow

  • Positive control
    • Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab110243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 5 - 15 µg/ml.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

Flow Cyt Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal).




ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB4 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B15 antibody
    • CI-B15 antibody
    • Complex I B15 subunit antibody
    • Complex I-B15 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody
    • NADH ubiquinone oxidoreductase B15 subunit antibody
    • NADH-ubiquinone oxidoreductase B15 subunit antibody
    • NDUB4_HUMAN antibody
    • NDUFB4 antibody
    see all


  • All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg

    Predicted band size: 15 kDa

  • Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
    Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.

  • HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.


This product has been referenced in:
  • Anand R  et al. Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells. PLoS One 11:e0160258 (2016). WB . Read more (PubMed: 27479602) »
  • Chang HY  et al. Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells. Cell Death Dis 5:e1540 (2014). IF ; Human . Read more (PubMed: 25429617) »
See all 12 Publications for this product

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Brain - Cerebellum)
Brain - Cerebellum
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

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