Product nameAnti-NDUFB5 antibody
See all NDUFB5 primary antibodies
DescriptionRabbit polyclonal to NDUFB5
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Gorilla
- WB: HEK-293T, Jurkat and MCF7 whole cell lysates; Mouse heart and kidney tissue lysates. IHC-P: Human kidney and spleen tissues. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab230215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 16,22 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFB5 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI SGDH antibody
- CI-SGDH antibody
- Complex I SGDH antibody
All lanes : Anti-NDUFB5 antibody (ab230215) at 1/500 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16,22 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human spleen tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NDUFB5 (green) using ab230215 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L).
ab230215 has not yet been referenced specifically in any publications.