Overview

  • Product name
    Anti-NDUFB6 antibody [21C11BC11]
    See all NDUFB6 primary antibodies
  • Description
    Mouse monoclonal [21C11BC11] to NDUFB6
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    NDUFB6 from cow

  • Positive control
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB6 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B17 antibody
    • CI antibody
    • CI B17 antibody
    • CI-B17 antibody
    • Complex I mitochondrial respiratory chain B17 subunit antibody
    • Complex I-B17 antibody
    • Complex IB17 antibody
    • MGC13675 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 6 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 antibody
    • NADH ubiquinone oxidoreductase B17 subunit antibody
    • NADH ubiquinone oxidoreductase beta subunit 6 antibody
    • NADH-ubiquinone oxidoreductase B17 subunit antibody
    • NDUB6_HUMAN antibody
    • NDUFB6 antibody
    see all

Images

  • All lanes : Anti-NDUFB6 antibody [21C11BC11] (ab110244) at 0.5 µg/ml

    Lane 1 : isolated mitochondria from Human heart at 5 µg
    Lane 2 : isolated mitochondria from cow heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 15 kDa

  • Overlay histogram showing HepG2 cells stained with ab110244 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110244, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Askeland G  et al. Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Sci Rep 8:9817 (2018). Read more (PubMed: 29959348) »
  • Khorsandi SE  et al. Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers. BMC Res Notes 10:547 (2017). Read more (PubMed: 29096719) »
See all 21 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:


ab14713
ab110244
ab14715
ab14745
ab14705

These have been tested in blue native gel. These antibodies are also frequently referenced in the literature for Blue Native. An example is a paper attached showing these in BNPAGE.

The other antibody, ab55521 has not been tested in blue native gel. We do produce many products in house, but also source many externally. Therefore all our antibodieswill have been tested in different ways. Therefore, if an antibody has been tested in western blot, this does not mean it has also been tested in blue native gel. In this case, if you have the same question regarding any of our other antibodies, please do not hesitate to contact us again and we will be happy to confirm the information for you.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to let me know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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